primary ucp1 antibody a5857  (ABclonal Biotechnology)

Journal: iScience

Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

doi: 10.1016/j.isci.2022.105769

Figure Lengend Snippet: Increased energy expenditure in Ccdc92 KO mice on HFD (A-D) Male Ccdc92 KO and littermate WT mice were fed HFD for 12 weeks (A) Food intake/BW, (B) total locomotor activity/BW, (C) O 2 consumption/BW, and (D) energy expenditure/BW were measured using the Comprehensive Lab Animal Monitoring System (CLAMS), WT: n = 5, Ccdc92 KO: n = 7. (E-H) Male Ccdc92 KO and littermate WT mice were fed HFD for 14 weeks (E) Representative images of H&E staining and quantification of adipocyte size in brown adipose tissue (BAT), n = 5/group. Scale bar = 50 μm (F) Representative images of IHC for UCP1 in BAT and quantitative analysis, n = 6/group. Scale bar = 50 μm (G) Representative immunoblot and quantification of UCP1, n = 4/group. (H) mRNA expression of lipid catabolism and thermogenesis-related genes in BAT was determined by RT-qPCR, n = 7-8/group. Data are presented as mean ± SEM, ∗p < 0.05; ∗∗p < 0.01. Analysis in A-D and H used two-way ANOVA with Bonferroni correction. Analysis in E, F, and G used unpaired two-tailed t -test.

Article Snippet: For immunostaining, mouse tissue sections embedded in paraffin were incubated with primary antibodies, including CD44 (BioLegend #103002, 1:200), CD68 (Santa Cruz #sc-20060, 1:200), Perilipin (Novus #NB100-60554, 1:200), or UCP1 (ABclonal, A5857, 1:200) overnight at 4°C, followed by incubation with a fluorescent-dye (Alexa Fluor 594- or Alexa Fluor 488; 1:1,000)-conjugated or enzyme (Alkaline phosphatase, 1:1,000)-conjugated secondary antibody.

Techniques: Activity Assay, Staining, Western Blot, Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: iScience

Article Title: Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

doi: 10.1016/j.isci.2022.105769

Figure Lengend Snippet:

Article Snippet: For immunostaining, mouse tissue sections embedded in paraffin were incubated with primary antibodies, including CD44 (BioLegend #103002, 1:200), CD68 (Santa Cruz #sc-20060, 1:200), Perilipin (Novus #NB100-60554, 1:200), or UCP1 (ABclonal, A5857, 1:200) overnight at 4°C, followed by incubation with a fluorescent-dye (Alexa Fluor 594- or Alexa Fluor 488; 1:1,000)-conjugated or enzyme (Alkaline phosphatase, 1:1,000)-conjugated secondary antibody.

Techniques: Derivative Assay, Virus, Recombinant, Lysis, Extraction, Immunoprecipitation, Magnetic Beads, Protease Inhibitor, Luciferase, SYBR Green Assay, Membrane, Bradford Assay, Saline, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, RNA Sequencing, Software

Journal: Nutrients

Article Title: L-Arabinose Elicits Gut-Derived Hydrogen Production and Ameliorates Metabolic Syndrome in C57BL/6J Mice on High-Fat-Diet

doi: 10.3390/nu11123054

Figure Lengend Snippet: Sequences of oligonucleotide primers targeting indicated genes related to lipid metabolism.

Article Snippet: Antibodies list: CPT1A (A5307, ABclonal, Wuhan, China), UCP1 (A5857, ABclonal), UCP3(A16996, ABclonal), PPARα (sc-9000, Santa Cruz, Dallas, TX, USA), PPARγ (2435S, CST, Danvers, MA, USA), FAS (3189S, CST), SREBP1 (Sc13551, Santa Cruz), PGC-1α (TA319007, Origene, Rockville, MD, USA), NDUFS3 (459130, Invitrogen, Waltham, MA, USA), SDHB (459230, Invitrogen), UQCRC1 (459140, Invitrogen), COX4 (459600, Invitrogen), ATP Synthase Subunit Alpha (459240, Invitrogen), SOD2 (sc-137254, Santa Cruz), β-Actin (3700S, CST), α-Tubulin (3873S, CST).

Techniques: Sequencing, Binding Assay